doi: 10.15389/agrobiology.2013.2.58eng
UDC 636.52/.58:573.6.086.83:636.082
INTEGRATION AND EXPRESSION OF MARKER GENES IN CHICKEN EMBRYOS WITH THE USE OF RETROVIRAL VECTOR
N.A. Volkova1, L.A. Volkova1, I.K. Fomin1, N.A. Zinovieva1, L.Sh. Gorelik2, N.S. Lotsmanova3
1All-Russian Research Institute of Animal Husbandry, Russian Academy of Agricultural Sciences,
pos. Dubrovitsy, Moscow Rrovince, 142132 Russia,
e-mail: natavolkova@inbox.ru;
2Ural State Academy of Veterinary Medicine,
13, ul. Gagarina, Troitsk, Chelyabinsk Province, 457100 Russia,
e-mail: natavolkova@inbox.ru;
3Base Medical College № 15 of Moscow Healthcare Department,
4a, ul. Shakuleva, Moscow, 109263 Russia,
e-mail: natavolkova@inbox.ru
Received January 19, 2013
The efficiency was investigated of transfer of exogenous DNA to chicken embryos in vivo with the use of retroviral vectors. The gene construction involves a marker gene GFT under control of promoter of yearly genes of human cytomegalovirus CMV IE (pLNCgfp) or promoter of Moloney leukemia virus Mo-MuLV (pLgfpSN). Two packing lines GP + envAM12 and PT67 were used for the delivery of retroviral vectors. It was established the high efficiency of embryo transformation with the use of pLNCgfp gene construction (up to 18.8 %). The expression of marker gene was demonstrated in cells of 5- and 15-day age chicken embryos.
Keywords: cell engineering, transgenesis, retrovirus vectors, chicken.
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