doi: 10.15389/agrobiology.2013.2.52eng
UDC 636.4:591.465.12:576.3/.7.086.83
MODELLING OF MATURATION SYSTEMS FOR PORCINE OOCYTE in vitro
T.I. Kuzmina1, D.A. Novichkova1, N.A.Volkova2
1All-Russian Research Institute of Farm Animal Genetics and Breeding, Russian Academy of Agricultural Sciences,
55а, Moskovskoe sh., St. Petersburg-Pushkin, 196601 Russia,
e-mail: prof.kouzmina@mail.ru, live8avis@mail.ru;
2All-Russian Research Institute of Animal Husbandry, Russian Academy of Agricultural Sciences,
pos. Dubrovitsy, Moscow Province, 142132 Russia,
e-mail: natavolkova@inbox.ru
Received January 15, 2013
A system of extracorporal maturation of growing and fully grown oocytes have been developed on the basis of research of a role of structural elements of porcine follicles in formation of mature oocytes and a preventive estimation of the functional status of oocytes (BCB-test). The cultivation of oocytes in the developed system allows to receive till 45 % of embryos at the stages of late morulae and blastocysts.
Keywords: cell technology of reproduction, pig, oocytes, embryos, fertilization in vitro, BCB-test.
REFERENCES
1. Niemann H., Kues W., Carnwath J.W. Transgenic farm animals: current status and perspectives for agriculture and biomedicine. In: Genetic engineering in livestock: Ethics of Science and Technology Assessment. Springer, Berlin, v. 34, 2009: 1-30.
2. Whyte J.J., Prather R.S. Genetic modifications of pigs for medicine and agriculture. Mol. Reprod. Dev., 2011, 78: 879-891.
3. Ernst L.K., Volkova N.A., Zinov'eva N.A. Sel’skokhozyaistvennaya Biologiya [Agricultural Biology], 2009, 2: 4-9.
4. Coy P., Romar R., Ruiz S., Cánovas S., Gadea J., Vázquez F., Matás C. Birth of piglets after transferring of in vitro-produced embryos pre-matured with R-roscovitine. Reproduction, 2005, 129(6): 747-755.
5. Hunter M. Oocyte maturation and ovum quality in pigs. Rev. Reprod., 2000, 5: 122-130.
6. Kuz'mina T.I., Al'm Kh.,Torner Kh. Metody polucheniya embrionov svinei in vitro [Methods of In Vitro Production of Pig Embryos]. St. Petersburg—Pushkin, 2008.
7. Kuz'mina T.I., Murza G.V., Matashina O.P., Novikova N.O. Genetika i selektsiya v zhivotnovodstve: vchera, segodnya, zavtra [Compilation of Sci. Works: Genetics and Selection in Animal Husbandry]. St. Petersburg—Pushkin, 2010: 207-212.
8. Kuz'mina T.I., Torner Kh., Al'm Kh. Dostizheniya nauki i tekhniki APK, 2010, 4: 66-68.
9. Tarkowski A. An air-drying method for chromosomal preparation from mouse eggs. Cytogenetics, 1966, 1: 394-400.
10. Yoshioka K., Suzuki C., Onishi A. Defined system for in vitro production of porcine embryos using a single basic medium. J. Reprod. Dev., 2008 Jun, 54(3): 208-213.
11. Tareq K.M., Akter Q.S., Khandoker M.A., Tsujii H. Selenium and vitamin E improve the in vitro maturation, fertilization and culture to blastocyst of porcine oocytes. J. Reprod. Dev., 2012, 19(58/6): 621-628.
12. Rodriguez-Gonzalez E., Lopez-Bejar M., Velilla E., Paramio M.T. Selection of prepubertal goat oocytes using the brilliant cresyl blue test. Theriogenology, 2002, 57: 1397-1409.
13. El Shourbagy S.H., Spikings E.C., Freitas M., St John J.C. Mitochondria directly influence fertilisation outcome in the pig. Reproduction, 2006 Feb, 131(2): 233-245.
