doi: 10.15389/agrobiology.2013.2.71eng

UDC 636.1/.2:599.723:575.174.015.3

ISSR-PCR AND MOBILE GENETIC ELEMENTS IN GENOMES OF FARM MAMMALIAN SPECIES

V.I. Glazko1, E.A. Gladyr'2, A.V. Feofilov1, N.V. Bardukov1, T.T. Glazko1

1Russian State Agrarian University – Moscow Timiryazev Agricultural Academy,
49, ul. Timiryazevskaya, Moscow, 127550 Russia,
e-mail: vglazko@yahoo.com;
2All-Russian Research Institute of Animal Husbandry, Russian Academy of Agricultural Sciences,
pos. Dubrovitsy, Moscow Province, 142132 Russia

Received January 11, 2013

 

Highly polymorphic ISSR-PCR markers, the DNA fragments  flanked by invert microsatellite repeats, are widely applied with the aim of the genomic scan (polyloci genotyping). The sequencing of such DNA fragments from domestic horse and cattle genomes testified the close connection between the inverted microsatellite repeats and products of recombination between endogenous retroviruses. Mutual genomic positioning of microsatellites and endogenous retroviruses as a source of polymorphisms of ISSR-PCR markers was discussed.

Keywords: microsatellites, retrotransposons, endogenous retroviruses, ISSR-PCR markers.

 

Full article (Rus)

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