doi: 10.15389/agrobiology.2019.1.19eng
UDC 635.21:632.4:631.524:577.21
Acknowledgements:
Supported financially by the Ministry of Education and Science of the Russian Federation (project No. 14.579.21.0012 of June 05, 2014, ID RFMEFI57914X0012), the used plant material is maintained within the framework of theme No. 0662-2019-0004 (VIR)
MULTIPLEX PCR-BASED IDENTIFICATION OF POTATO GENOTYPES AS DONORS IN BREEDING FOR RESISTANCE TO DISEASES AND PESTS
E.V. Rogozina1, E.V. Terentjeva2,3, E.K. Potokina1, E.N. Yurkina1,
A.V. Nikulin2, Ya.I. Alekseev3,4
1Federal Research Center Vavilov All-Russian Institute of Plant Genetic Resources, 42-44, ul. Bol’shaya Morskaya, St. Petersburg, 190000 Russia, e-mail rogozinaelena@gmail.com (✉ corresponding author), e.potokina@yahoo.com,
2All-Russian Research Institute of Agricultural Biotechnology, 42, ul. Timiryazevskaya, Moscow, 127550 Russia, e-mail elena-terentev@inbox.ru, nikylin_a@list.ru;
3LLC Syntol, 42, ul. Timiryazevskaya, Moscow, 127550 Russia, e-mail jalex@syntol.ru;
4Institute for Analytical Instrumentation RAS, 31-33, ul. Ivana Chernyh, St. Petersburg, 198095 Russia, e-mail jalex@syntol.ru
ORCID:
Rogozina E.V. orcid.org/0000-0002-2743-068X
Yurkina E.N. orcid.org/0000-0002-4513-194X
Terentjeva E.V. orcid.org/0000-0003-2777-0948
Nikulin A.V. orcid.org/0000-0002-8197-7694
Potokina E.K. orcid.org/0000-0002-2578-6279
Alekseev Ya.I. orcid.org/0000-0002-1696-7684
Received July 18, 2018
The breeding of potatoes with the traditional technology of hybridization and selection of individual plants is a time-consuming process. The use of DNA markers linked to genes underlying resistance to diseases and pests can significantly improve the efficiency of the selection of valuable genotypes in the early stages of breeding process. The aims of the work were i) screening of potato genetic resources from the VIR collection (Vavilov All-Russian Institute of Plant Genetic Resources, St. Petersburg) for the presence of genes encoding resistance to cyst-forming nematodes, potato wart, potato viruses X and Y (PVX and PVY) by the multiplex PCR method; ii) evaluation of the effectiveness of molecular markers for the identification of potato genotypes resistant to the golden nematode, potato wart and PVY. A total of 90 accessions from the VIR collection were studied, among them the cultivated potatoes from two subspecies, the S. tuberosum subsp. chiloense (native varieties of Chile) and S. tuberosum subsp. tuberosum (breeding varieties), as well as hybrid clones have been distinguished as sources and donors of potato resistance to pathogens of the economically significant or quarantine diseases. In this work, several molecular markers that were early recommended for the identification of potato genes responsible for the resistance to cyst nematodes, Y and X viruses, and potato wart were first used for the multiplex PCR analysis of genetically diverse material. Ten markers used were TG 689, 57 R, N 195 of H1 gene and Gro1-4-1 of Gro1-4 gene (resistance to the golden nematode Globodera rostochiensis pathotypes Ro1, Ro4), marker Gpa 2-2 of Gpa2 gene (resistance to the pale nematode G. pallida pathotype Pa2), RYSC3 marker of Ryadg gene, Ry 186 marker of Rychc gene and YES3-3A marker of Rysto gene (all genes provide immunity to the potato virus Y), the PVX marker of the Rx gene (immunity to potato virus X) and the NL 25 marker of the Sen1 for resistance to potato wart caused by Synchytrium endobioticum (Schilb.) Percival. The PCR screening results were matched with the phenotypic characteristics of the test potato genotypes for resistance to the golden nematode, wart and potato virus Y. Multiplex PCR analysis allowed us to identify potato genotypes with several (up to five) resistance genes, including those providing resistance to the nematode G. rostochiensis patotype Ro1, S. endobioticum patotype 1 and potato virus Y. A significant association was established between the molecular markers linked to the H1 gene and the resistance of potato genotypes to the nematode G. rostochiensis Ro1 (rA = 0.59, rs = 0.72-0.79), and between the marker N L25 of Sen1 gene and potato resistance to wart (rA = 0.62). No association was detected between Ryadg and Rysto molecular markers and plant resistance to potato virus Y due to a large number of tested resistant potato genotypes which possibly carry unknown immunity genes.
Keywords: potato, Solanum ssp., interspecific hybrids, DNA markers, marker assisted selection, potato wart, Synchytrium endobioticum, nematodes, Globodera rostochiensis Ro1, potato virus Y.
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