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Sel’skokhozyaistvennaya Biologiya [Agricultural Biology], 2019, Vol. 54, № 1, p. 110-120.
(how to cite this article)

doi: 10.15389/agrobiology.2019.1.110eng

UDC 632.937:632.937.33:575.21

Acknowledgements:
The studies were performed according to the State Task No. 075-00376-19-00 of the Ministry of Science and Higher Education of the Russian Federation within the framework of scientific research work on the subject No 0686-2019-0009

 

BIOLOGICAL CHARACTERIZATION, PHENOTYPIC AND GENOTYPIC STRUCTURE OF PREDATORY STINKBUG Perillus bioculatus Fabr. (Heteroptera, Pentatomidae) POPULATION IN KRASNODAR REGION

V.Ya. Ismailov1, I.S. Agas’eva1, V.I. Kil2, E.V. Fedorenko1,
M.V. Nefedova1,E.N. Besedina1

1All-Russian Research Institute of Biological Plant Protection, PO box 39, Krasnodar, 350039 Russia, e-mail vlyaism@yandex.ru, agasieva5@yandex.ru, vlkil@inbox.ru, ms.fedor1960@mail.ru, dollkaSneba@yandex.ru, katrina7283@yandex.ru (✉ corresponding author);
2Kuban State University, 149, ul. Stavropolskaya, Krasnodar, 350040 Russia, e-mail vlkil@inbox.ru (✉ corresponding author)

ORCID:
Ismailov V.Ya. orcid.org/0000-0002-6713-0059
Fedorenko E.V. orcid.org/0000-0003-4411-626X
Agas’eva I.S. orcid.org/0000-0002-1216-1106
Nefedova M.V. orcid.org/0000-0001-5390-233X
Kil V.I. orcid.org/0000-0002-3762-9019
Besedina E.N. orcid.org/0000-0002-9976-5614

Received March 20, 2018

 

Perillus bioculatus Fabr. (1775) is a predatory bug in the family Pentatomidae, a prospective North American entomophage of the Colorado potato beetle. Earlier, Colorado potato beetle eggs were deemed the most preferable for the predator feeding and the main obstacle for perillus acclimatization on new territories. However, in monitoring perillus populations that have acclimatized in several areas of Krasnodar region for the last decade, we revealed the predators’ feeding on ragweed leaf beetle (Zygogramma suturalis Fabr.) larvae and ragweed cutworm Tarachidia candefacta Hübn. (Noctuidae: Lepidoptera) caterpillars and also on all growth stages of Colorado potato beetle and other insects. In Krasnodar Krai perillus can give annually three generations and is present in three phenoforms, with red-black, orange-black and white-black scutellum. The abundance of P. bioculatus is influenced by feeding conditions and by other entomophages, e.g. egg-eaters of genus Trissolcus (Hymenoptera: Scelionidae) and tachina flies (Diptera: Tachinidae). Analysis of scutellum color inheritance in seven different crosses, including reciprocal ones, between red, orange (or yellow) and white parents showed that the F1 and F2 progeny from all combinations, including white individuals crossed with each other, have only red and orange shield (1:1). PCR analysis of P. bioculatus bugs in F2 from crossing insects of different phenoforms revealed that the bugs with the same phenoform in F2 are genetically different from each other for the frequency of certain RAPD markers. These data indicate non-Mendelian inheritance of scutellum coloration trait in perillus that additionally attract attention to the point. RAPD (random amplification of polymorphic DNA)- and ISSR(inter simple sequence repeat)-PCR analysis detected intra- and inter-population variation of the predatory bugs molecular genetic structure from different zones of the Krasnodar Krai. PCR analysis with almost all used specific primers revealed statistically significant differences (p = 0.05) in molecular genetic structure and DNA polymorphism for the populations studied. Geographic populations from the village of Staro-Nizhesteblievskaya and the city of Krasnodar (Krasnodar Krai) were genetically close while there genetic identities with the Moldavian village (Crimean region, Krasnodar Krai) population were low. This comports with their geographical location and indicates geographic variability of P. bioculatus populations’ genetic structure. The researches executed by us confirm plasticity of this species and prospects for its further acclimatization within Colorado potato beetle area.

Keywords: predatory bugs, Perillus bioculatus Fabr., adventive species, acclimatization, PCR analysis, molecular genetic similarity, Colorado beetle, entomophages, biocontrol.

 

 

REFERENCES

  1. Sablon L., Dickens J.C., Haubruge É., Verheggen F.J. Chemical ecology of the Colorado potato beetle, Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae), and Potential for Alternative Control Methods. Insects, 2013, 4: 31-54 CrossRef
  2. Lawrence S.D., Novak N.G., Ju C.J., Cooke J.E. Potato, Solanum tuberosum, defense against Colorado potato beetle, Leptinotarsa decemlineata (Say): microarray gene expression profiling of potato by Colorado potato beetle regurgitant treatment of wounded leaves. J. Chem. Ecol., 2008, 34(8): 1013-1025 CrossRef
  3. Hare J.D. Ecology and management of the Colorado potato beetle. Annu. Rev. Entomol., 1990, 35: 81-100 CrossRef
  4. Hare J.D. Impact of defoliation by the Colorado potato beetle on potato yields. J. Econ. Entomol., 1980, 73: 369-373 CrossRef
  5. Babenko A.S., Shternshis M.V., Andreeva I.V., Tomilova O.G., Korobov V.A. Entomofagi v zashchite rastenii [Entomophagous species in control of plant pests]. Novosibirsk, 2001 (in Russ.).
  6. Sukhoruchenko G.I., Dolzhenko V.I., Novozhilov K.V. Vestnik zashchity rastenii, 2006, 1: 3-12 (in Russ.).
  7. Sumarokov A.M. Vosstanovlenie bioticheskogo potentsiala biogeotsenozov pri umen'shenii pestitsidnykh nagruzok [Restoration of the biotic potential of biogeocenoses by reducing pesticide loads]. Donetsk, 2009 (in Russ.).
  8. Tanskii V.I. Fitosanitarnaya ustoichivost' agrobiotsenozov [Agro-biocenotic phytosanitary resistance]. St. Petersburg, 2010 (in Russ.).   
  9. Listopadova E.S., Nefedova M.V., Agas'eva I.S. Mezhdunarodnyi nauchno-issledovatel'skii zhurnal, 2014, 2(21-2): 10-11 (in Russ.).
  10. Dolzhenko T.V., Kozlova E.G., Dolzhenko O.V. Rossiiskaya sel'skokhozyaistvennaya nauka, 2016, 2-3: 21-23 (in Russ.).
  11. Clausen C.P. Introduced parasites and predators of arthropod pests and Weed. World review. Washington, 1978.
  12. Jasič I. On the life cycle of Perillus bioculatus (Heteroptera, Pentatomidae) in Slovakia. Acta Ent. Bohemoslovaca, 1975, 72(6): 383-390.
  13. Wegorek W.G., Pruszynski S. Stan badan nad introdukcija do Polsky wrogow naturalnych stronki ziemnisczanej (Leptinotarsa decemlineata Say). Post Nauk Roln., 1979, 5(26): 61-73.
  14. Gusev G.V. Entomofagi koloradskogo zhuka [Entomophages of Colorado potato beetle]. Moscow, 1991 (in Russ.).
  15. Jermy T. The introduction of Perillus bioculatus into Europa to control the Colorado beetle.  EPPO Bulletin, 1980, 10(4): 475-479 CrossRef
  16. Pruscynsky S., Wegorek W. Researchers on biology and introduction of Podisus maculiventris (Say) — new for Poland predator of the Colorado potato beetle (Leptinotarsa decemlineata Say). Mat. 22-j Ses. nauk; Inst. ochr. rosl. Poznan, 1981: 127-136.
  17. De Clercq P. Spined soldier bug, Podisus maculiventris Say (Hemiptera: Pentatomidae: Asopinae). In: Encyclopedia of entomology. J.L. Capinera (ed.). Springer, Heidelberg, 2008, V. 4: 3508-3510.
  18. Rabitsch W. True bugs (Hemiptera, Heteroptera). Chapter 9.1. Alien terrestrial arthropods of Europe. A. Roques, M. Kenis, D. Lees, C. Lopez-Vaamonde, W. Rabitsch, J.-Y. Rasplus, D. Roy (eds.). BioRisk, 2010, 4: 407-433 CrossRef
  19. Rabitsch W. Alien true bugs of Europe (Insecta: Hemiptera: Heteroptera). Zootaxa, 2008, 1827: 1-44.
  20. Wegorek P. Insecticide resistance management strategy for Colorado potato beetle (Leptinotarsa decemlineata Say) in Poland. Resistant Pest Management Newsletter, 2002, 11(2): 22-30.
  21. Alyokhin A., Baker M., Mota-Sanchez D., Dively G., Grafius E. Colorado potato beetle resistance to insecticides. Am. J. Potato Res., 2008, 85(6): 395-413 CrossRef
  22. Alyokhin A. Colorado potato beetle management on potatoes: current challenges and future prospects. Fruit Vegetable and Cereal Science and Biotechnology, 2009, 3: 10-19. 
  23. Bishop B.A., Grafius E.J. Insecticide resistance in the Colorado potato beetle. In: Chrysomelidae biology. P.H.A. Jolivet, M.L. Cox (eds.). SBP Academic Publishing, Amsterdam, The Netherlands, 1996, V. 1: 355-377.
  24. Grafius E.J. Is local selection followed by dispersal a mechanism for rapid development of multiple insecticide resistance in the Colorado potato beetle? American Entomologist, 1995, 41(2): 104-109 CrossRef
  25. Alyokhin A., Dively G., Patterson M., Castaldo C., Rogers D., Mahoney M., Wollam J. Resistance and cross-resistance to imidacloprid and thiamethoxam in the Colorado potato beetle Leptinotarsa decemlineata. Pest Manag Sci. 2007, 63(1): 32-41 CrossRef
  26. Mota-Sanchez D., Hollingworth R.M., Grafius E.J., Moyer D.D. Resistance and cross-resistance to neonicotinoid insecticides and spinosad in the Colorado potato beetle, Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae). Pest Manag. Sci., 2006, 62(1): 30-37 CrossRef
  27. Scott I.M., Tolman J.H., MacArthur D.C. Insecticide resistance and cross-resistance development in Colorado potato beetle Leptinotarsa decemlineata Say (Coleoptera: Chrysomelidae) populations in Canada 2008-2011. Pest Manag. Sci., 2015, 71(5): 712-721 CrossRef
  28. Neimorovets V.V. Priroda, 2016, 2: 43-48 (in Russ.).
  29. Agas'eva I.S., Nefedova M.V. Evraziiskii soyuz uchenykh (ESU), 2015, 6(5): 90-93 (in Russ.).
  30. Kil' V.I., Ismailov V.Ya., Agas'eva I.S. Rossiiskaya sel'skokhozyaistvennaya nauka, 2012, 3: 30-33 (in Russ.).
  31. Agas'eva I.S., Ismailov V.Ya., Nefedova M.V. Trudy Kubanskogo gosudarstvennogo agrarnogo universiteta,2014, 3(48): 27-30 (in Russ.).
  32. Agas'eva I.S., Ismailov V.Ya., Nefedova M.V., Fedorenko E.V. The species composition and bioregulatory activity of entomophages in potato pest control system. Agricultural Biology, 2016, 51(3): 401-410 CrossRef (in Engl.).
  33. Voronin K.E., Pukinskaya G.A., Isak I.V., Asekyan B.P., Voronina E.G., Bondarenko N.V., Ryakhovskii V.V., Dyadechko N.P., Yasnosh V.A. Metodicheskie ukazaniya po ispol'zovaniyu kriteriev effektivnosti prirodnykh populyatsii entomofagov i entomopatogenov /Pod redaktsiei K.V. Novozhilova [How to use performance criteria of natural populations of entomophages and entomopathogens: practical guide. K.V. Novozhilov (ed.)]. Moscow, 1991 (in Russ.).
  34. Yeh F.C., Yang R.C., Boyle T.B.J., Ye Z.H., Mao J.X.  POPGENE, the user-friendly shareware for population genetic analysis. Computer program and documentation. Molecular Biology and Biotechnology Centre, University of Alberta, Edmonton, 1997.
  35. Dospekhov B.A. Metodika polevogo opyta [Methods of field trials]. Moscow, 1985 (in Russ.).   
  36. Derjanschi V., Elisoveţcaia D., Calestru L. Ploşniţa Perillus bioculatus F. (Heteroptera, Pentatomidae) — prădătorul principal al gândacului de Colorado. Akademos, 2013, 4(31): 90-93.
  37. Derjanschi V., Elisoveţcaia D. Predatory shield bug Perillus bioculatus F. (Hemiptera, Pentatomidae) in the Republic of Moldova: acclimatization or natural colonization? Proc. Int. Conf. of Zooloists «Actual problems of protection and sustainable use of the animal world diversity». Chisinau, 2013, Iss. 8: 124-125.
  38. Shagov E.M. Vliyanie temperatury na khishchnogo klopa Perillus bioculatus Fabr. (Hemiptera: Pentatomidae). Zoologicheskii zhurnal, 1968, XLVII(4): 563-570.
  39. Fent M., Aktaç N. Die Verbreitung des Perillus bioculatus (Fab.) (Heteroptera: Pentatomidae: Asopinae) im türkischen Teil Thrakiens. Heteropteron, 2007, 25: 7-10.
  40. Coudron T.A., Kim Y. Life history and cost analysis for continuous rearing of Perillus bioculatus (Heteroptera: Pentatomidae) on a zoophytogenous artificial diet. Biological and Microbial Control. J. Econ. Entomol., 2004, 3: 807-812 CrossRef
  41. Kivan M. Some observations on Perillus bioculatus (F.) (Heteroptera: Pentatomidae) a new record for the entomofauna of Turkey. Türkish Journal of Entomology, 2004, 28(2): 95-98.
  42. Onder F., Karsavuran Y., Tezcan S., Fent M. Heteroptera (Insecta) catalogue of Turkey. Meta Basým Matbaacýlýk Hizmetleri, Ýzmir, 2006. 
  43. Simov N., Langourov M., Grozeva S., Gradinarov D. New and interesting records of alien and native true bugs (Hemiptera: Heteroptera) from Bulgaria. Acta zoologica bulgarica, 2012, 3(64): 241-252.
  44. Proti L.J., Živi N. Perillus bioculatus (Fabricius) (Heteroptera: Pentatomidae) in Serbia. Acta entomologica serbica, 2012, 17(1/2): 23-28.

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