Sel’skokhozyaistvennaya Biologiya [Agricultural Biology], 2018, Vol. 53, ¹ 1, p. 85-95.
(how to cite this article)

doi: 10.15389/agrobiology.2018.1.85eng

UDC 633.11:632.4.01:575.174

Acknowledgements:
Supported financially by Russian Science Foundation (project ¹ 14-26-00067 for molecular genetic characterization of the pathogen and project ¹ 16-16-10005 for identification of wheat resistance genes using PCR markers)

 

GENETIC STRUCTURE OF RUSSIAN AND KAZAKHSTANI LEAF RUST
CAUSATIVE AGENT Puccinia triticina Erikss. POPULATIONS AS ASSESSED
BY VIRULENCE PROFILES AND SSR MARKERS

E.I. Gultyaeva1, E.L. Shaydayuk1, V.P. Shamanin2, À.Ê. Àkhmetova3,
V.A. Tyunin4, E.R. Shreyder4, I.V. Kashina5, L.A. Eroshenko6,
G.A. Sereda7, A.I. Morgunov3

1All-Russian Research Institute of Plant Protection, Federal Agency for Scientific Organizations,3, sh. Podbel’skogo, St. Petersburg, 196608 Russia, e-mail eigultyaeva@gmail.com (✉ corresponding author), eshaydayuk@bk.ru;
2Stolypin Omsk State Agricultural University, 1, Institutskaya pl., Omsk, 644008 Russia, e-mail vp.shamanin@omgau.org;
3International Maize and Wheat Improvement Center (CIMMYT), P.Ê. 39, Emek, 06511, Ankara, Turkey, e-mail aigkur@gmail.com, a.morgounov@cgiar.org;
4Ñhelyabinsk Research Institute of Agriculture, Federal Agency for Scientific Organizations, p. Timiryazevskii, Chebarkul Region, Chebarkul Province, 456404 Russia, e-mail chniisx2@mail.ru, shreyder11@mail.ru;
5OOO Agrocomplex Kurgansemena, 57-203, ul. Volodarskogo, Kurgan, 640000 Russia, e-mail irfom45@mail.ru;
6Pavlodar Research Institute of Agriculture, 32, ul. 60 let Oktyabrya, p. Krasnoarmeika, Pavlodar Province, 140000 Republic of Kazakhstan, e-mail selektion2011@mail.ru;
7Karaganda Research Institute of Plant Growing and Breeding, s. Tsentralnoe, Bukhar-Zhyrauskii Region, Karaganda Province, 110435 Republic of Kazakhstan,
e-mail 12345680@bk.ru

ORCID:
Gultyaeva E.I. orcid.org/0000-0001-7948-0307
Shreyder E.R. orcid.org/0000-0001-6094-962x
Shaydayuk E.L. orcid.org/0000-0003-3266-6272
Kashina I.V. orcid.org/0000-0001-6753-7855
Shamanin V.P. orcid.org/0000-0003-4767-9957
Eroshenko L.A. orcid.org/0000-0001-5722-1507
Àkhmetova À.Ê. orcid.org/0000-0003-2957-8239
Sereda G.A. orcid.org/0000-0002-0593-5839
Tyunin V.A. orcid.org/0000-0002-3609-0488
Morgunov A.I. orcid.org/0000-0001-7082-5655

Received June 29, 2017

 

Leaf rust caused by Puccinia triticina Erikss is an economically significant disease of spring wheat in the West-Asian Russia and Northern Kazakhstan. Successful wheat breeding for leaf rust resistance necessitates characterization of Lr gene effectiveness, the impact of new wheat varieties on the pathogen virulence, and isolation between populations of P. triticina. Until now, nobody used a uniform infectious material in P. triticina population study, as it was collected from a different set of varieties in each region. Thence, the virulence assessment data were significantly influenced by the effects of host plants. We were the first to compare the structure of P. triticina populations in the West-Asian Russia and Northern Kazakhstan on virulent and molecular genetic properties, using as a source of infectious material a single set of the wheat samples dated 2016 that were tested there within the framework of the Kazakhstan-Siberian Spring Wheat Improvement Network Program (KASIB). Ninety one single-pustule isolates have been tested in the virulence analysis, including 13 isolates from Chelyabinsk, 28 from Omsk, 6 from Kurgan, 16 from Akmolinskaya, 16 from Pavlodarskaya, and 12 from Karagandinskaya provinces. Eleven phenotypes of virulence have been identified with the use of 20 isogenic Thatcher lines with Lr genes (TcLr). The similar phenotypes were found on cultivars Duat, Tertsia, Omskaya 35, Pamyati Azieva, Saratovskaya 29, Chebarkulskaya 3 and line Eritrosperum 85-08. The Russian pathotypes found on variety Astana and lines Lutescens 1003 and Lutescens 6/04-4 differed from Kazakhstan ones in virulence to TcLr11, and those colonizing variety GVK 2074/4 and line Lutescens 715 differed in avirulence to TcLr18. More significant differences in the virulence rang between regional populations have been observed on the line Lutescens 34/08-1 and the variety Rodnik. All studied P. triticina isolates were avirulent to TcLr19, TcLr24 and virulent to TcLr3a, TcLr3bg, TcLr3ka, TcLr14a, TcLr14b, TcLr16, TcLr17, TcLr30. The variation in virulence frequencies was observed on the wheat lines with the genes Lr1, Lr2a, Lr2b, Lr2c, Lr9, Lr15, Lr18, Lr20, and Lr26. Virulence to Lr9 was found in the pathogen populations collected from the wheat samples Duet, Tertsia, Chebarkulskaya 3, Lutescens 34/08-19 carrying this gene. The differences in populations on virulence were found using the indices of genetic distances Nei D and Fst, the analog for binary data in AMOVA. The Nei index values indicated a high similarity between the majority of the Northern-Kazakhstani and Russian populations, except of those from Chelyabinskaya and Karagandinskaya provinces. According to the Fst index, the high similarity was found between the populations from Omsk, Kurgan and Northern Kazakhstan. The Chelyabinsk population appeared to be close to the Kurgan but varied from others. In the test, a total of 46 isolates and 9 genotypes have been identified using 12 SSR markers. In this, 21 polymorphic alleles were found in the studied set. The Nei and Fst indices revealed the moderate differences between the Chelyabinsk and Karaganda populations and a high similarity between other populations studied. The current survey defined the high similarity of leaf rust pathogen populations in West-Asian Russia and Northern Kazakhstan, assuming the existence of a single fungus population in the studied territories. In order to prevent the P. triticina epiphytoties on the adjacent territories of Russia and Kazakhstan, the constant updating of wheat varieties and higher genetic diversity are strongly recommended. In addition, the varieties should be grown according to the «mosaic distribution» scheme using optimal areas for genetically homogeneous varieties.

Keywords: Puccinia triticina Erikss., leaf rust, common wheat, populations, virulence, Lr-genes, SSR markers.

 

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REFERENCES

  1. Shamanin V.P., Petukhovskii S.L. Vestnik Omskogo gosudarstvennogo agrarnogo universiteta, 2012, 4(8): 4-9 (in Russ.).  
  2. Belan I.A., Rosseeva L.P., Meshkova L.V., Blokhina N.P., Pershina L.A., Trubocheeva N.V. Vestnik Altaiskogo gosudarstvennogo agrarnogo universiteta, 2017, 1(147): 5-14 (in Russ.).  
  3. Koishybaev M. Zashchita i karantin rastenii, 2015, 9: 21-26 (in Russ.).  
  4. Kolmer J.A., Kabdulova M.G., Mustafina M.A., Zhemchuzhina N.S., Dubovoy V.Russian populations of Puccinia triticina in distant regions are not differentiated for virulence and molecular genotype. Plant Pathol., 2015, 64(2): 328-336 CrossRef
  5. Shamanin V., Salina E., Wanyera R., Zelenskiy Y., Olivera P., Morgounov A. Genetic diversity of spring wheat from Kazakhstan and Russia for resistance to stem rust Ug99. Euphytica, 2016, 121(2): 287-296 CrossRef
  6. Tyunin V.A., Shreider E.R., Gul'tyaeva E.I., Shaidayuk E.L. Vavilovskii zhurnal genetiki i selektsii. 2017, 21(5): 523-529 CrossRef (in Russ.).  
  7. D'yakov Yu.T. Populyatsionnaya biologiya fitopatogennykh gribov [Population biology of phytopathogenic fungi]. Moscow, 1998 (in Russ.).  
  8. Kolmer J.A., Hanzalova A., Goyeau H., Bayles R., Morgounov A. Genetic differentiation of the wheat leaf rust fungus Puccinia triticina in Europe. Plant Pathol.,2013, 62: 21-31 CrossRef
  9. McCallum B., Ceto-Coh P., Xue A. Physiologic specialization of Puccinia triticina, the causal agent of wheat leaf rust, in Canada in 2010. Can. J. Plant Pathol., 2016, 38(4): 440-447 CrossRef
  10. Gul'tyaeva E.I., Aristova M.K., Shaidayuk E.L., Kazartsev I.A. Mikologiya i fitopatologiya, 2017, 51(1): 54-59 (in Russ.).  
  11. Kolmer J. Leaf rust of wheat: pathogen biology, variation and host resistance. Forests, 2013, 4(1): 70-84 CrossRef
  12. Kolmer J.A., Ordoñez M.E. Genetic differentiation of Puccinia triticina populations in Central Asia and the Caucasus. Phytopathology, 2007, 97(9): 1141-1149 CrossRef
  13. Mikhailova L.A., Gul'tyaeva E.I., Mironenko N.V. Metody issledovanii struktury populyatsii vozbuditelya buroi rzhavchiny pshenitsy Ruccinia recondita Rob. ex Desm. f. sp. tritici [Methods for studying structure of populations of the causative agent of wheat brown rust Ðuccinia recondita Rob. ex Desm. f. sp. tritici].St. Petersburg, 2000 (in Russ.).   
  14. McIntosh R.A., Wellings C.R., Park R.F. Wheat rusts. An atlas of resistance genes. Plant Breeding Institute, University of Sydney, CSIRO, Australia, 1995.
  15. Long D.L., Kolmer J.A.A North American system of nomenclature for Puccinia recondita f. sp. tritici. Phytopathology, 1989, 79(5): 525-529.
  16. Justesen A.F., Ridout C.J., Hovmøller M.S. The recent history of Puccinia striiformis f. sp. tritici in Denmark as revealed by disease incidence and AFLP markers. Plant Pathol., 2002, 51: 13-23 CrossRef
  17. Duan X., Enjalbert J., Vautrin D., Solignac M., Giraud T. Isolation of 12 microsatellite loci, using an enrichment protocol, in the phytopathogenic fungus Puccinia triticina. Mol. Ecol. Notes, 2003, 3(1): 65-67 CrossRef
  18. Szabo L.S., Kolmer J.A. Development of simple sequence repeat markers for the plant pathogenic rust fungus Puccinia triticina. Mol. Ecol. Notes, 2007, 7(4): 708-710 CrossRef
  19. Serfling A., Krämer I., Lind V., Schliephake E., Ordon F. Diagnostic value of molecular markers for Lr genes and characterization of leaf rust resistance of German winter wheat cultivars with regard to the stability of vertical resistance. Eur. J. Plant Pathol., 2011, 130(4): 559-575 CrossRef
  20. Sibikeev S.N., Markelova T.S., Druzhin A.E., Vedeneeva M.L., Singkh D. Doklady RASKHN, 2011, 2: 3-5 (in Russ.).  
  21. Mikhailova L.A. Genetika vzaimootnoshenii vozbuditelya buroi rzhavchiny i pshenitsy /Pod redaktsiei M.M. Levitina [Genetics of the relationship between the causative agent of brown rust and wheat plants. M.M. Levitin (ed.)]. St. Petersburg, 2006 (in Russ.).  
  22. Pavlova T.V., Mikhailova L.A. Mikologiya i fitopatologiya, 1997, 31(5): 60-66 (in Russ.).  
  23. Volkova G.V. Zashchita i karantin rastenii, 2010, 9: 13-17 (in Russ.).  
  24. Berlyand-Kozhevnikov V.M., Dmitriev A.P., Budashkina E.B., Shitova I.T., Reiter V.G. Ustoichivost' pshenitsy k buroi rzhavchine (geneticheskoe raznoobrazie populyatsii griba i rasteniya-khozyaina) [Wheat resistance to brown rust (genetic diversity of populations of fungus and host plants)]. Novosibirsk, 1978 (in Russ.).  
  25. Lebedev V.B. Rzhavchina pshenitsy v Nizhnem Povolzh'e [Wheat rust disease in the Lower Volga Region]. Saratov, 1998 (in Russ.).    
  26. Sorokina G.K. Dinamika rasovogo sostava i spetsializatsiya vozbuditelya buroi rzhavchiny pshenitsy (Ruccinia recondita Rob. ex Desm. f. sp. tritici Erikss. et Henn.) na territorii evropeiskoi chasti SSSR. Avtoreferat kandidatskoi dissertatsii [Dynamics of race composition and specialization of causative agent of wheat brown rust (Ðuccinia recondita Rob. ex Desm. f. sp. tritici Erikss. et Henn.) on the territory of the European part of the USSR. PhD Thesis]. Moscow, 1978 (in Russ.).   
  27. Turapin V.P., Mostovoi V.A. Rzhavchinnye bolezni zernovykh kul'tur v Respublike Kazakhstan i bor'ba s nimi [Rust diseases of grain crops and phytopathological control in the Republic of Kazakhstan]. Almaty, 1995 (in Russ.).
  28. Koishibaev M.K. Bolezni zernovykh kul'tur: simptomy, rasprostranenie i vredonosnost', spetsializatsiya, biologicheskie osobennosti, struktura populyatsii vozbuditelei i integrirovannaya zashchita posevov [Diseases of cereal crops: symptoms, occurrence and harmfulness, specialization, biological features of pathogens and the structure of populations, integrated crop protection]. Almaty, 2002 (in Russ.).

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