doi: 10.15389/agrobiology.2014.1.32eng
UDC 581.143.6:57.08
OPTIMIZATION OF MEDIA FOR in vitro PLANT CULTIVATION BY THE EXAMPLE OF WATER SORREL (Rumex aquaticus L.)
M.V. Skaptsov, D.V. Balabova, M.G. Kutsev
Altai State University, South Siberian Botanical Garden,
61, prosp. Lenina, Barnaul, 656049 Russia,
e-mail: mr.skaptsov@mail.ru, m_kucev@mail.ru, biotech@bio.asu.ru
Received May 21, 2012
Accumulation of polyphenols makes a real problem under in vitro plant propagation because of toxic effects of phenol oxidation products to callus tissue, and in some cases a frequent subculturing is required. We screened the possible media components for decreasing negative effects of polyphenols to cell proliferation and viability under cultivation, callus tissue of Sorrel aqueous Rumex aquatics L. used as a model. Callus was induced from leaf explants on basal Murashige and Skoog medium with benzyladenin-6 and α-naphthylacetic acid. Derived callus was subcultivated on the same medium with the addition of sorbents (polyvinylpyrrolidone) and antioxidants (sodium thiosulfate). There is the prolongation of life of callus without frequent subculturing by reducing accumulation of polyphenols in the tissues. Through quantitative studies of the amount of polyphenols by a modified method of Folin-Chokalteu, it was established that the concentration of phenolic compounds in the tissues in the classical media with long-term cultivation made up to 7 mg per 1 g of callus tissue, if re-counted to gallic acid, followed by a growth arrest and cell death. In contrast, in the modified media a content of polyphenols is an average of up to 2 times lower than in the callus tissue grown in the medium without use of adsorbents and antioxidants.
Keywords: Rumex aquaticus L., callus formation, plant biotechnology, polyvinylpyrrolidone, polyphenols.
REFERENCES
1. Ai-fang Y., Su-yun J., Xiao-guang D., Ju-ren Z. High efficient in vitro propagation and salt tolerant plantlet regeneration in Rumex. Acta Pratacultural Sinica, 2005, 14(2): 56-61.
2. Naz S., Ali A., Iqbal J. Phenolic content in vitrocultures of chick pea (Cicer arietinum L.) during callogenesis and organogenesis. Pac. J. Bot., 2008, 40(6): 2525-2539.
3. Lewandowski I., Kahnt G. Development of a tissue culture system with unemerged inflorescence of Miscanthus «Gigantheus» for the induction and regeneration of somatic emrbryoids. Beiträge zur Biologie der Pflanzen, 1993, 1: 439-451.
4. Sapers G. Chitosan enhances control of enzymatic browning in apple and pear juice by filtration. J. Food Science, 1992, 57: 1192-1193. CrossRef
5. Walker J. Enzymatic browning in foods. Its chemistry and control. Food Technology New Zeland, 1977, 12: 19-25.
6. Cilliers J., Singleton V. Caffeic acid autooxidation and the effects of thiols. J. Agriculture and Food Chemistry, 1990, 38: 1789-1796. CrossRef
7. Butenko R.G. Biologiya kletok vysshikh rastenii in vitro i biotekhnologiya na ikh osnove: uchebnoe posobie [In vitro cell biology and biotechnology of higher plants]. Moscow, 1999.
8. Murashige T. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant., 1962, 15(13): 473-497.
9. Muratova S.A., Shornikov D.G., Yankovskaya M.B., Papikhin R.V. Sovremennoe sadovodstvo, 2010, 1: 96-100.
10. Vechernina N.A. Biotekhnologiya rastenii: uchebnoe posobie [Plant biotechnology]. Barnaul, 2009.
11. Rukovodstvo po metodam kontrolya kachestva i bezopasnosti biologicheski aktivnykh dobavok k pishche. Rukovodstvo. R 4.1.1672-03 (Utv. glavnym gosudarstvennym sanitarnym vrachom RF 30.06.2003)/MZ Rossii [Guidelines for control of biologically active food additives]. Moscow, 2004.
