doi: 10.15389/agrobiology.2013.1.53eng
UDC 635.649:631.522/.524:577.2
AFLP-ANALYSIS OF VARIETAL POLYMORPHYSM IN Capsicum annuum L.
E.A. Snigir'1, 2, O.N. Pyshnaya1, E.Z. Kochieva2, N.N. Ryzhova2
1All-Russian Research Institute of Breeding and Seed Production of Vegetable Crops, Russian Academy of Agricultural Sciences,
pos. VNIISSOK, Odintsovo Region, Moscow Province, 143080 Russia,
e-mail: pishnaya_o@mail.ru;
2Center “Bioengineering”, Russian Academy of Science,
7/1, prosp. 60-letiya Oktyabrya, Moscow, 117312 Russia
e-mail: ekochieva@yandex.ru
Received May 12, 2012
AFLP-system of multipoint marking was used for estimation of genetic diversity in 45 varieties of Capsicum annuum L. As a result, it was selected 8 primer combinations, which were the most informative for marking of paprika genome. Due to these primers the authors obtained the specific DNA-spectrums for each variety. In total, 956 polymorphous AFLP-fragments were detected, of which 182 fragments characterize the individual varietal genotypes. The calculated coefficients of intervarietal genetic diversity are varying from 0.005 to 0.064. On dendrogram all varieties form general cluster with weak intrinsic differentiation that indicates the low degree of genetic polymorphism. So the forming of distance basal branches, revealed in closely related cultural species C. frutescens, C. chinense and C. baccatum, reflects the potential of their genetic diversity which may be used in paprika breeding.
Keywords: Capsicum annuum, genetic diversity, genetic polymorphism, AFLP-markers.
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