doi: 10.15389/agrobiology.2013.1.61eng

UDC 632.78:577.21


V.I. Kil', E.N. Besedina

All-Russian Research Institute of Biological Plant Protection, Russian Academy of Agricultural Sciences,
VNIIBZR, Krasnodar, 350039 Russia,

Received September 19, 2011


The results of the PCR analysis of two codling moth populations using RAPD and SSR markers are presented. The molecular genetic structure of investigated pest populations is described and its variability under influence of insecticides, varying climatic conditions during 2008 to 2011 and geographic location is studied. The intra-population genetic diversity by two microsatellite loci was estimated in pests from the gardens with different insecticide press. Genetic diversity of codling moth populations was shown to depend mainly on genetic features of the populations, bur not on the insecticide load or weather conditions.

Keywords: polymorphism, genetic diversity, microsatellite loci, population, codling moth, DNA, RAPD-PCR, SSR-PCR.


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1. Scott  L.J.,  Lawrence  N.,  Lange  C.L. et al. Population dynamics and gene flow of Helicoverpa armigera (Lepidoptera: Noctuidae) on cotton and grain crops in the Murrumbidgee Valley, Australia. J. Econ. Entomol., 2006, 99: 155-163.
2. Endersby  N.M.,  Hoffmann  A.A.,  McKechnie  S.W.,  Weeks  A.R.Is there genetic structure in populations of Helicoverpa armigera from Australia? Entomol. Exp. Appl., 2007, 122: 253-263.
3. Timm  A.E.,  Geertsema  H.,  Warnich  L. Gene flow among Cydia pomonella (L.) (Lepidoptera: Tortricidae) geographic and host populations in South Africa. J. Econ. Entomol., 2006, 99: 341-348.
4. Franck  P.one_pix,  Reyes  M.,  Olivares one_pix J.,  Sauphanor  Bone_pix. Genetic architecture in codling moth populations: comparison between microsatellite and insecticide resistance markers. Mol. Ecol., 2007, 16: 3554-3564.
5. Calkins  C.O.,  Faust  R.J. Overview of areawide programs and the program for suppression of codling moth in the western USA directed by the United States Department of Agriculture — Agricultural Research Service. Pest. Manag. Sci., 2003, 59: 601-604.
6. Kil'  V.I.,  Besedina  E.N.,  Fedicheva  O.O. Trudy Stavropol'skogo otdeleniya Russkogo entomologicheskogo obshchestva (materialy II Mezhdunarodnoi nauchno-prakticheskoi internet-konferentsii «Aktual'nye voprosy entomologii») [Sci. Works of the Stavropol’ Department of Russian Entomological Society. Proc. II Int. Sci.-Pract. Internet Conf. «Live Issues of Entomology»], 2009, 5: 154-157.
7. Kil'  V.I.,  Besedina  E.N.,  Fedicheva  O.O. Informatsionnyi byulleten' Vostochno-Palearkticheskoi regional'noi sektsii Mezhdunarodnoi organizatsii po biologicheskoi bor'be s vrednymi zhivotnymi i rasteniyami (materialy dokladov Mezhdunarodnogo simpoziuma «Zashchita rastenii — dostizheniya i perspektivy», g. Kishinev) [Inf. Bull. of the East-Palearctic Regional Department of the International Organization for Biological Control of Pest Animals and Plants. Proc. Int. Symp. «Crop Protection – Achievments and Prospects»], 2009, 40: 92-94.
8. Kil'  V.I. Materialy Mezhdunarodnoi nauchno-prakticheskoi konferentsii «Biologicheskaya zashchita rastenii — osnova stabilizatsii agroekosistem» [Proc. Int. Sci.-Pract. Conf. «Biological Crop Protection – A Basis of Sustainable Agroecosystems»]. Krasnodar, 2010, v. 6: 281-284.
9. Kil'  V.I. Politematicheskii setevoi elektronnyi nauchnyi zhurnal Kubanskogo gosudarstvennogo universiteta [Multitopic Scientific Electronic Network Magazine of Kuban State University], 2010, 62(08), oktyabr' (
10. Kil'  V.I. Metodika otsenki DNK-polimorfizma populyatsii nasekomykh s pomoshch'yu PTSR (RAPD- i ISSR-PCR): metodicheskie rekomendatsii [The Method for Evaluating DNA Polymorphism in Insect Populations Using RAPD- and ISSR-PCR: Guidelines]. Krasnodar, 2009.
11. Kil'  V.I. Politematicheskii setevoi elektronnyi nauchnyi zhurnal Kubanskogo gosudarstvennogo universiteta [Multitopic Scientific Electronic Network Magazine of Kuban State University], 2011, 68(04), aprel' (
12. Kil'  V.I.,  Gronin  V.V.,  Krutenko  D.V.,  Ismailov  V.Ya. Sel’skokhozyaistvennaya Biologiya [Agricultural Biology],  2008, 1: 70-76.
13. Franck  P.,  Guérin  F.,  Loiseau  A.,  Sauphanor  B.Isolation and characterization of microsatellite loci in the codling moth Cydia pomonella L. (Lepidoptera, Tortricidae). Molecular Biology Notes, 2005, 5(1): 99-102.
14. Asser-Kaiser  S.,  Fritsch  E.,  Undorf-Spahn K.,  Kienzle J.,
Eberle  K.E.,  Gund  N.A.,  Reineke  A.,  Zebitz  C.P.W.,  Heckel D.G.,  Huber  J.,  Jehle  J.A. Rapid emergence of Baculovirus resistance in codling moth due to dominant sex linked inheritance. Science, 2007, 317: 1916-1918.