УДК 631.461.5:575
ESTIMATION OF PHENOTYPIC PRESENTATIONS OF BACTERIAL GENES, CONTROLLING THE EFFICIENCY OF NITROGEN-FIXING SYMBIOSIS WITH PLANTS
O.P. Onishchuk, N.A. Provorov, N.I. Vorob’ev, B.V. Simarov
The collection of recombinant species of nodule bacterium of lucerne (Sinorhizobium meliloti) with additional copies of dicarboxylic acid transport gene (process, limiting nitrogen fixation) was used and the authors shown, that the symbiosis efficiency is limiting by the plant inability to absolute involvement of biological nitrogen in growth process and the transfer to above-ground organs nitrogen transport forms, accumulation of which in roots and nodules inhibits an energy entry in bacteroides. On the data of factor analysis of vegetative experiment results the amplification succinate permease dctA structure gene in complex with specific transcription regulator dctBD increases the symbiosis efficiency regardless of plant variety and vegetation conditions, but the amplification of nifA and ntrA genes (nonspecific regulators of dctA gene) required for the increase of N2-fixing activity only at unfavorable for it conditions.
Keywords: symbiotic nitrogen fixation, nodule bacteria, leguminous plants, genetic construction, dicarboxylic acids, factorial analysis, symbiotic efficiency, competitiveness, ecologically safe soil tillage.
All-Russia Research and Development Institute of Agricultural Microbiology, RAAS, St.Petersburg – Pushkin-8 196608, Russia e-mail: provorov@newmail.ru |
Received January 11, 2010
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