УДК 633.16:57.086.83
COMPARATIVE EVALUATION CALLUSOGENESIS AND REGENERATION IN DIFFERENT BARLEY VARIETIES
V.E. Chernov, G.I. Pendinen
The authors studied the features of callusogenesis and regeneration (number, mass, morphological characteristics and efficiency of callus formation, morphological characteristics of regenerants and regeneration efficiency) in the species from different section of Hordeum L. genus. For induction of callusogenesis the three explants types were used (young blossom cluster, immature corcule, ripe corn seeds), and also the three nutrient media differed on mineral content (N6, B5L, Murashige-Skoog). It was established, that a formation of callus tissue in species of Hordeum section differs from the same in species of different section (Anisolepis, Critesion, Stenostachys). The callus type do not depends on medium content and used explants. Some of studied species didn’t revealed the regeneration of plants from callus. The single regeneration type was specific for species from Hordeum section, but the multiple type — for species from Anisolepis, Critesion, Stenostachys sections. The type regeneration didn’t depend on selected explants (young blossom cluster, immature corcule, and ripe corn seed).
Keywords: callusogenesis, regeneration, explants, in vitro culture, genus Hordeum, wild barley.
N.I. Vavilov All-Russia Research and Development Institute of Plant Growing, RAAS, St.Petersburg 190000, Russia, |
Received January 15, 2009 |
